TABLE 3.
Primers used for RT-qPCR
| Primer | Target (species, gene, primer orientation) | Sequence (5′–3′) |
|---|---|---|
| XSH0367 | C. thermocellum, PPi-pfk, forward | ATGCATATTCGGACAATCC |
| XSH0905 | C. thermocellum, PPi-pfk, reverse | TGAGCTGCTCCGTAAACTGC |
| XSH0901 | T. saccharolyticum, ATP-pfk, forward | TAGAGACACGGCAACGTCAC |
| XSH0902 | T. saccharolyticum, ATP-pfk, reverse | ATAATCTCTGCTCCTCCAGC |
| XSH0198 | C. thermocellum, recA, forward | TTTACGGCCAGGGTATTTCA |
| XSH0199 | C. thermocellum, recA, reverse | GCCAATCTTCTGACCGTTGT |
| XSH0200 | T. saccharolyticum, recA, forward | GAAGCCTTAGTGCGAAGTGG |
| XSH0201 | T. saccharolyticum, recA, reverse | GAAGTCCAACATGTGCATCG |