Skip to main content
. 2022 Oct 26;88(22):e01258-22. doi: 10.1128/aem.01258-22

TABLE 3.

Primers used for RT-qPCR

Primer Target (species, gene, primer orientation) Sequence (5′–3′)
XSH0367 C. thermocellum, PPi-pfk, forward ATGCATATTCGGACAATCC
XSH0905 C. thermocellum, PPi-pfk, reverse TGAGCTGCTCCGTAAACTGC
XSH0901 T. saccharolyticum, ATP-pfk, forward TAGAGACACGGCAACGTCAC
XSH0902 T. saccharolyticum, ATP-pfk, reverse ATAATCTCTGCTCCTCCAGC
XSH0198 C. thermocellum, recA, forward TTTACGGCCAGGGTATTTCA
XSH0199 C. thermocellum, recA, reverse GCCAATCTTCTGACCGTTGT
XSH0200 T. saccharolyticum, recA, forward GAAGCCTTAGTGCGAAGTGG
XSH0201 T. saccharolyticum, recA, reverse GAAGTCCAACATGTGCATCG