Patterns in the atom% 13C enrichment of metabolites identified as compatible solutes differed with respect to carbon source. Trends in major compatible solutes differed among compound types, with ectoine highly enriched during the metabolism of vanillin. Carbon sources were aggregated by response into three classes: soluble (glucose, xylose, amino acids, lactic acid, glycerol, and oxalic acid), insoluble (cellulose and palmitic acid), and vanillin. Points represent average atom% 13C values. Error bars correspond to standard deviations among triplicates. In most cases, error bars are narrow and obscured by the data point, excluding day 14 data, where only a single sample was taken for all carbon sources except for oxalate and cellulose.