Patterns in atom% 13C enrichment of metabolites identified as disaccharides differed with respect to carbon source. The proportion of source-derived 13C occurring in disaccharides (maltose, trehalose, or cellobiose) steadily increased during the degradation of insoluble compounds. Maltose, trehalose, and cellobiose overlapped in m/z and could not be distinguished using our chromatography conditions. Values represent the averages of triplicates. Carbon sources were aggregated by response into two classes: soluble (glucose, xylose, amino acids, lactic acid, glycerol, and oxalic acid) and insoluble (cellulose and palmitic acid). Error bars correspond to standard deviations among triplicates. In most cases, error bars are narrow and obscured by the data point, excluding day 14, where only a single sample was taken for all carbon sources except for oxalate and cellulose.