Fig. 5.

Verification of 4977 bp deletion. In the 4977 bp deletion sequencing peak diagram, the red box shows that the repeated sequence at both ends of 4977 bp deletion is located in region 8470–8482 at one end and region 13447–13459 at the other end (A). The second well from the left is Marker DL2000, the third well is negative control, and the fourth well to the tenth well is 4977 bp missing band of 7 samples. The results above are the second amplification product of nested PCR (B).