FIG. 1.

Schematic presentation of the engineered PIgphage immunogen. Oligonucleotides coding for the PT1 epitope (underlined) were inserted by PCR assembly into the CDRs of the Ig V_H domain and cloned into the phagemid vector as a fusion with cpIII (see Materials and Methods). The correct PCR assembly was verified by DNA sequencing, and the sequence of the PT1 epitope, inserted into the CDR1 loop, is shown. Indicated in boldface letters are the alanine and tryptophan residues from the FR1 and FR2 regions, respectively. FR, immunoglobulin heavy-chain variable-domain framework region.