TABLE 2.
Plate coated with | Response when incubated with:
|
|||||
---|---|---|---|---|---|---|
Normal serum | Positive serum | Anti-PIgphage | Anti-NF | PIgphage | NF | |
PT1 | 0.184 | 0.248 | 0.135 | 0.168 | ||
TAg | 0.554 | 2.258 | 0.469 | 0.547 | ||
PIgphage | 0.484 | 0.366 | 1.856 | 1.720 | ||
Normal serum | 0.289 | 0.525 | ||||
Anti-PIgphage | 1.624 | 1.627 | ||||
Anti-TAg | 0.247 | 0.533 | ||||
Anti-NF | 1.929 | 1.855 |
The presence of specific antibodies was measured by ELISA as described in Materials and Methods. Sera from T. crassiceps-infected and noninfected mice were used as positive and negative controls, respectively. Phage particles (PIgphage and NF) were used at 2 × 109. Sera used were diluted 1:20. Anti-PIgphage, anti-TAg, and anti-NF are pooled sera from mice immunized with the corresponding antigen (five mice in each group).