Figure 4.

Instant 3D three-color live-cell imaging of a COS-7 cell. Maximal intensity projections of (a) connexin (stained with GFP) imaged through the green channel, (b) nuclei (stained with mCherry) imaged through the orange channel, (c) mitochondria (stained with MitoTracker Deep Red) imaged through the deep red channel, and (d) the merged image are shown. (e) Time series of connexin dynamics indicated in the white dashed square in (d) in four frames in which colors encode the height of the sample. (f) The same time points as (e) but showing the mitochondria dynamics. (g) Temporal evolution of the connexin (upper panel) and mitochondria mobility (lower panel) in 0.3 s (see color bar). Scale bars in (a) represent 10 μm and in (e) represent 3 μm.