Figure 5.

Example ACF analyses of independent HeLa cells irradiated at different excitation powers. Fluorescence images of Dronpa-C12-labeled actin in live HeLa cells, with ROI used in analyses highlighted, are shown in top part of figure. Corresponding computed ACFs from ROIs (points) and respective simultaneous fits (lines) are shown in bottom part of figure. The time-lag ranges for the fits varied from τ = 2 to either τ = 10 or τ = 20. Time windowing was done with T_W = 100 frames. Pixel size is 177.78 nm. Frame time is 10 ms. Image series length is 50 s. Scale bars: 5 μm.