Table 2.
Comparison of fitted and simulated parameters
| Fit | Simulation | |
| D (pixels2 frame−1) | 2.80 ± 0.08 | 3 |
| K (frame−1) | 0.86 ± 0.01 | 0.9 |
| ρon | 0.38 ± 0.03 | 0.44 |
| fD | 0.26 ± 0.02 | 0.3 |
The parameters are for fits shown Fig. 3. Simulation had photobleaching rate kp = 10−4 frame−1 and was generated on 128 × 128 pixel grid with 10,744 total particles and T =2048 frames. Fitted parameters and errors were obtained by splitting the simulation spatially into five 64 × 64 ROIs, with some overlap between different parts, and then calculating the mean and its SE from their analyses. The ROIs were chosen so that a significant portion of the simulated dendritic structure was encompassed overall.