a. qPCR of canonical cell type markers for endothelial (Cldn5, Abcb1a, Mfsd2a), mural (Pdgfrb, Acta2, Myh11), astrocytes (Aqp4, Aldh1l1), oligodendrocytes (Mog), neurons (Rbfox3), and microglia (Aif1) from mouse cortex, n=3, two-tailed t test, *p < 0.01, **p < 0.001, ***p < 0.0001,****p < 0.00001, n.s. = not significant. b. Immunofluorescence of blood vessels enriched from mouse cortex using the BVE protocol. Scale bar, 20μm