Figure 4. Exogeneous cell control with ON- and OFF- switches.
A. Representative ON-switch incorporating an NS3 protease to control the CAR activity. CAR will be stabilized only under the presence of a drug that can inhibit the protease activity to induce the T cell activation. B. Representative OFF-switch using the zinc finger degron motif and a synthetic ubiquitin ligase. A drug that can induce the dimerization between the degron and the ligase will degrade the CAR, so the T cells cannot be activated under the presence of the drug.