Fig 2. Lung targeting of 833c&Freso but not fresolimumab.

After bleomycin treatment, rats were injected iv with radio-iodinated antibodies ¹²⁵I-833c&Freso, ¹²⁵I-833cX&Freso, and ¹²⁵I-fresolimumab (see Methods). (A, C, E) SPECT/CT images of rats taken at 1* and 24 h. Maximum intensity projection (MIP), coronal, sagittal, and transverse cross sections are shown. (B, D, F) Planar F067-scintigraphy imaging at the indicated timepoints. A-F: N = 2–3 rats per group (G) Biodistribution analysis of radioiodinated antibodies. Radioactivity was counted in the indicated organs excised from bleomycin-exposed rats. Uptake was quantified as the percentage of injected dose per gram tissue (means ± SD). N = 3–10 rats per group. *Imaging started at 30 min and takes approximately 30 to 90 min.