TABLE 1.
Apoptosis and necrosis induced by the treatment of SVC1 cells for 7 days with different porin concentrationsa
| Cell treatment with porin (μg/ml) | Apoptotic cellsb (%) | Necrotic cellsb (%) |
|---|---|---|
| None (control) | 2 ± 1 | 0 |
| 1 | 10 ± 3 | 0 |
| 5 | 30 ± 5 | 2 ± 1 |
| 10 | 70 ± 4 | 3 ± 1 |
| 15 | 65 ± 7 | 11 ± 3 |
| 30 | 55 ± 5 | 29 ± 2 |
Experimental details are reported in Materials and Methods.
The percentage of apoptotic and necrotic cells was evaluated by the acridine orange method. The data are reported as means ± standard errors of the mean of determinations performed in triplicate on three different samples.