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. 2022 Nov 8;11:e82709. doi: 10.7554/eLife.82709

Figure 8. Sloth1 and Sloth2 physically interact with complex III and regulate its assembly.

Figure 8.

(A–B) Blue native PAGE gel of mitochondria isolated from (A) 10 adult thoraxes and (B) 10 whole 3rd instar larvae of indicated genotype. Bands corresponding to native respiratory complexes are indicated with arrowheads. (C–D) SDS-PAGE and western blotting of mitochondria isolated from (C) adult thorax and (D) whole 3rd instar larvae of indicated genotype. Each lane loaded equal amount of protein (15 µg). Blots were stripped and reprobed after detection of each antigen. (E–F) Western blots from co-immunoprecipitation experiments in transfected S2R+ cells using Sloth1-FLAG and Sloth2-FLAG as bait and either (E) RFeSP-HA or (F) CG10075-HA as prey. Blots were striped and reprobed after detection of each antigen. Arrowheads indicated expected band, asterisks indicate unknown bands.