FIG. 1.

T. cruzi trypomastigotes induce β-chemokine expression in murine macrophages. Total RNA was extracted from thioglycolate-elicited peritoneal macrophages (A and C) cultured in the presence of medium (M) alone or with T. cruzi trypomastigotes (Tc) in a parasite/cell ratio of 1:1 for 6 h at 37°C in a humidified chamber containing air plus 5% CO₂. Total RNA was extracted from C3H/HeJ peritoneal cells (B) harvested from normal (N) mice and from mice injected 6 h before with 10⁵ T. cruzi trypomastigote forms. cDNA was synthesized, and PCR was performed with primers for the β-chemokines and for β-actin. Equal amounts of DNA were loaded into each well. The results shown are representative of five different experiments.