FIG. 6.

β-Chemokine-mediated cytostatic effects are reverted with l-NMMA. Thioglycolate-elicited murine macrophages were incubated with culture-derived T. cruzi trypomastigotes in a parasite/cell ratio of 1:1 for 2 h, and the extracellular parasites were removed. This was followed by 48 h of incubation, with or without recombinant murine MIP-1α (A), MIP-1β (B), RANTES (C), or JE/MCP-1 (D) (all at 100 ng/ml) and with or without l-NMMA (LN; 200 μM), PT (30 ng/ml), antibodies (ab) against MIP-1α (A), MIP-1β (B), RANTES (C), JE/MCP-1 (D) (all at 100 μg/ml), or irrelevant antibody (immunoglobulin G). After 48 h, the cultures were washed, fixed, and stained with Giemsa stain. Intracellular amastigotes were counted in 500 cells (at ×400 magnification under a light microscope). The data (means ± standard deviations) are representative of two independent experiments.