FIG. 7.

Effects of β-chemokines on control of T. cruzi replication in macrophages. Thioglycolate-elicited C3H/HeJ macrophages were infected with culture-derived trypomastigotes in a parasite/host cell ratio of 1:1 (A) or 5:1 (B and C) for 2 h, and the extracellular parasites were removed. MIP-1α, MIP-1β, RANTES, JE/MCP-1 (all at 100 ng/ml) or IFN-γ (100 U/ml) (A) or JE/MCP-1 (0, 1, 10, and 100 ng/ml) and/or IFN-γ (0, 1, 5, and 25 U/ml) (B and C) were then added to the cultures. The cells were incubated at 37°C in a humidified chamber containing 5% CO₂. The released parasites were counted daily (A) or on days 4 (B) and 6 (C) in a Newbauer chamber. The bars represent means ± standard deviations of triplicate counts. The data are representative of two independent experiments.