Figure 12.

The ERα inhibitor MPP inhibits the expression of apoptosis-related proteins in HIBD neonatal mice pretreated with ICA. The expression levels of (a) p53, (b) PUMA, (c) Bax, (d) Bcl-2, (e) caspase-3, and (f) cleaved caspase-3 in the cerebral ischemic penumbra (small black box in the upper left brain section) in MPP-treated HIBD newborn mice detected by tissue immunofluorescence, n = 8 mice in each group. Bar = 100 μm. (g) Representative western blot images and (h–l) quantitative analysis. (m) Quantitative analysis of the detected caspase-3 activity. ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 compared to the HI + ICA + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.