Figure 13.

Effects of the ERβ inhibitor PHTPP on the ERβ and ERα levels in HIBD newborn mice pretreated with ICA. The expression levels of (a) ERβ and (b) ERα in the cerebral ischemic penumbra (small black box in the upper left brain section) in PHTPP-treated HIBD newborn mice detected by immunofluorescence, n = 8 mice in each group. Bar = 100 μm. (a) Representative western blot images and (d, e) quantitative analysis. ^∗∗∗∗P < 0.0001 compared to the HI + ICA + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.