Figure 15.

Effects of the ERβ inhibitor PHTPP on autophagy-related proteins in HIBD neonatal mice pretreated with ICA. The expression of (a) Beclin1, (b) LC3, and (c) p62 in the cerebral ischemic penumbra (black box of the brain slice in the upper left corner) in neonatal HIBD mice treated with PHTPP detected by fluorescence, n = 8 mice in each group. Bar = 100 μm. (d) Representative western blot images and (e–g) quantitative analysis. ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 compared to the HI + ICA + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.