Figure 2.

Effect of ICA pretreatment on autophagy in HIBD newborn mice. The expression levels of (a) Beclin1, (b) LC3, and (c) p62 in the cerebral ischemic penumbra (IP, the small black box in the upper left brain slice; the neurons in this area are in a state of electrical failure, and the focus on saving these cells is the key and hot spot for the treatment of HIBD) of neonatal HIBD mice pretreated with ICA were detected by immunofluorescence, n = 8 mice in each group. Bar = 100 μm. (d) Representative western blot images and (e) quantitative analysis. ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 compared to the sham group,^##P < 0.01 and ^####P < 0.0001 compared to the HI + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.