Figure 3.

Effects of the autophagy inhibitor 3-MA on autophagy-related proteins in HIBD neonatal mice pretreated with ICA. Immunofluorescence detection of the expression of (a) Beclin1, (b) LC3, and (c) p62 in the ischemic penumbra (small black box in the upper left brain slice) of 3-MA-treated HIBD neonatal mice, n = 8 mice in each group. Bar = 100 μm. (d) Representative western blot images and (e–g) quantitative analysis. ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 compared to the HI + ICA + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.