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Effect of the autophagy inhibitor 3-MA on the level of apoptosis in HIBD neonatal mice pretreated with ICA. The numbers of TUNEL-positive cells (green fluorescence in the lower panel), NeuN-positive cells (red fluorescence), and FJC-positive neurons (green fluorescence in the upper panel) in the cerebral ischemic penumbra (small black box in the upper left brain slice) in 3-MA-treated HIBD newborn mice detected by tissue immunofluorescence, n = 8 mice in each group. Bar = 100 μm.
