Figure 8.

Effect of ICA pretreatment on ERα and ERβ levels in neonatal mice with HIBD. Immunofluorescence was used to detect the expression of (a) ERα and (b) ERβ in the ischemic penumbra of HIBD neonatal mice pretreated with ICA (the small black frame area in the upper left corner of the brain slice), n = 8 mice in each group. Bar = 100 μm. (c) Representative western blot images and (d, e) quantitative analysis. ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 compared to the sham group, ^####P < 0.0001 compared to the HI + Vehicle group. Data are presented as the mean ± SDs, n = 6 mice in each group.