Figure 1.

Single-cell mass cytometry reveals distinct immune composition between placental endovasculature and peripheral blood

(A) Our setup distinguishes maternal from fetal immune cells and their localization in the vasculature of the MFI.

(B and C) Composite UMAP of maternal immune cells in the MFI and PB across E10.5–E18.5, n = 26 mice. (C) Scaled cellular median intensity of lineage markers.

(D) Scaled median expression of protein markers used for Leiden clustering across maternal immune cells. First column represents cell type.

(E) Distribution of maternal immune cells across TIS, EV, and PB projected onto composite UMAP as contour plot.

(F) Fraction of immune cells relative to total in each compartment. Aggregated embryonic days.

(G) LDA based on maternal immune cell fractions in each compartment. Each dot represents a sample.

(H) Bray-Curtis dissimilarity based on maternal immune cell fractions in each compartment.