REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Biotin (Clone 1D4-C5)	BioLegend	Cat#409002
CD45 (Clone 30-F11)	BioLegend	Cat#103102
CD45.2 (Clone 104)	BioLegend	Cat#109802
Ter119 (Clone TER-119)	BioLegend	Cat#116202
B220 (Clone RA3-6B2)	BioLegend	Cat#103202
Ly-6G (Clone 1A8)	BioLegend	Cat#127602
CD11c (Clone N418)	BioLegend	Cat#117302
TCRβ (Clone H57-597)	BioLegend	Cat#109202
CD115 (Clone AFS98)	BioLegend	Cat#135502
CD69 (Clone H1.2F3)	BioLegend	Cat#104502
F4/80 (Clone BM8)	BioLegend	Cat#123102
CD3 (Clone 17A2)	BioLegend	Cat#100202
IgD (Clone 11-26c.2a)	BioLegend	Cat#405702
CD19 (Clone 6D5)	BioLegend	Cat#115502
CD25 (Clone 3C7)	BioLegend	Cat#101902
CD64 (Clone X54-5/7.1)	BioLegend	Cat#139302
CD80 (Clone 16-10A1)	BioLegend	Cat#104702
CD8 (Clone 53–6.7)	BioLegend	Cat#100702
CD11b (Clone M1/70)	BioLegend	Cat#101202
CD40 (Clone HM40-3)	BioLegend	Cat#102902
IgM (Clone RMM-1)	BioLegend	Cat#406502
CD117 (Clone 2B8)	BioLegend	Cat#105804
TCRγδ (Clone GL3)	BioLegend	Cat#118101
CTLA-4 (Clone UC10-4B9)	BioLegend	Cat#106302
Ly-6C (Clone HK1.4)	BioLegend	Cat#128002
CD194 (Clone 2G12)	BioLegend	Cat#131202
CD62L (Clone MEL-14)	BioLegend	Cat#104402
PD-L1 (Clone 10F.9G2)	BioLegend	Cat#124302
FcεRI-α (Clone MAR-1)	BioLegend	Cat#134302
CD335 (Clone 29A1.4)	BioLegend	Cat#137602
Siglec-F (Clone E50-2440)	BD Biosciences	Cat#552125
CD49b (Clone DX5)	BioLegend	Cat#108902
CD44 (Clone IM7)	BioLegend	Cat#103002
CD4 (Clone RM4-5)	BioLegend	Cat#100506
PD-1 (Clone 29F.1A12)	BioLegend	Cat#135202
MHC-II (Clone M5/114.15.2)	BioLegend	Cat#107602
CD86 (Clone GL-1)	BioLegend	Cat#105002
CD45.1 (Clone A20)	BioLegend	Cat#110702
FoxP3 (Clone FJK-16s) −158Gd	Fluidigm Sciences	Cat#3158003A
CD68 (Clone FA-11)	BioLegend	Cat#137002
RPS6 Ser235/Ser236 (Clone A17020B)	BioLegend	Cat#608602
CD31 (Clone MEC13.3)	BioLegend	Cat#102501
Chemicals, peptides, and recombinant proteins
Poly(I:C) LMW	Invivogen	Cat#tlrl-picw
Iododeoxyuridine	Sigma-Aldrich	Cat #I7125
Cisplatin	Sigma Aldrich	Cat#P4394
Critical commercial assays
K2EDTA evacuated blood collection tubes	Fisher Scientific	Cat#02-683-99A
RBC Lysis Buffer	BioLegend	Cat#420301
Accutase	Sigma Aldrich	Cat##SCR005
FOXP3 Transcription Factor Staining Buffer Set	eBioscience	Cat#00-5523-00
MaxPar X8 Antibody Labeling kit	Fluidigm Sciences	Cat#201300
TruStain FcX	BioLegend	Cat#101320
Cell-ID Intercalator-Ir	Fluidigm Sciences	Cat#201192
M.O.M. Immunodetection Kit	Vector Laboratories	Cat#BMK-2202
Deposited data
Raw mass cytometry data	This study, Mendeley Data	https://doi.org/10.17632/45gz4r28s2.1
Experimental models: Organisms/strains
Mouse: B6	The Jackson Laboratory	RRID: IMSR_JAX:000664
Mouse: B6.SJL-Ptprca Pepcb/BoyJ	The Jackson Laboratory	RRID: IMSR_JAX:002014
Oligonucleotides
Sex genotyping forward primer: 5-CTGAAGCTTTTGGCTTTGAG-3′	This study	N/A
Sex genotyping reverse primer: 5-CCGCTGCCAAATTCTTTGC-3′	This study	N/A
Software and algorithms
R	R Core Team, 2022	r-project.org
Python	N/A	python.org
ParkerICI/premessa	N/A	https://github.com/ParkerICI/premessa
Cydar	Lun et al., 2017	bioconductor.org/packages/release/bioc/html/cydar.html
gee	N/A	=CRAN.R-project.org/package = gee
limma	Ritchie et al., 2015	bioconductor.org/packages/release/bioc/html/limma.html
CellEngine	CellCarta, Montreal, Canada	cellengine.com
Single Cell Debarcoder	Zunder et al. 2015	github.com/nolanlab/single-cell-debarcoder
BioRender	BioRender	biorender.com
Scanpy	Wolf et al., 2018	scanpy.readthedocs.io
SciKit Learn	Pedregosa et al., 2011	scikit-learn.org
scikit-bio	The scikit-bio development team	scikit-bio.org
SciPy	Virtanen et al., 2020	scipy.org
statsmodels	Seabold and Perktold, 2010	statsmodels.org
Other
Microarray gene expression of developing mouse decidua and placenta	Knox and Baker, 2008	GEO: GSE11220
Immune associated genes	Immport	immport.org/resources
Vascular development gene dataset	The Jackson Laboratory	GO:0001944