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. 2022 Nov 15;41(7):111646. doi: 10.1016/j.celrep.2022.111646

Figure 1.

Figure 1

Age-related increase of pTau in THY-Tau22 mice

(A) Immunoreactivity for AT8 (pTau+, green) in coronal sections containing the hippocampal formation at different ages (months old, mo). Calbindin (CB, red). Right, enlarged views of distal CA1 (dCA1) and dorsal prosubiculum (ProS). Widefield epifluorescence.

(B) Normalized intensity measurements of AT8 immunoreactivity (mean, standard error) at six different age ranges in ProS and dorsal CA1 (CA1d). Age ranges (left to right): young (pink), 2–3 months (n = 2) and 5 months (n = 2); aging (green), 8–10 months (n = 2) and 14 months (n = 1); aged (purple), 16 months (n = 2), 18–19 months (n = 2), and 22–23 months (n = 2).

(C) Immunoreactivity for pTau (green) in deep cortical layers at 20 months relative to CB (cyan), parvalbumin (PV, magenta) and Purkinje Cell Protein 4 (PCP4, yellow)-immunopositive subpopulations.

(D) Lack of pTau in the medial septum (MS) in a 23-month tg mouse (case TT21D, female). Processes containing pTau found mostly within lateral septum (LS). Left, epifluorescence tile showing PV (magenta) and Special AT-Rich Sequence-Binding Protein 1 (SATB1; yellow) restricted to the MS. Right, enlarged view; note AT8 (green) mostly in LS.

Scale bars: 500 μm (A, left; C, left; D, left); 200 μm (A, right); 100 μm (D, right). Cases in (A) (top to bottom; mouse, sex): TT114A, male (m); TV132, m; TV143, m; TV136, female (f); TV139, m; TT33G, f. See also Figures S1 and S2.