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. 2022 Nov 15;41(7):111646. doi: 10.1016/j.celrep.2022.111646

Figure 2.

Figure 2

Preferential accumulation of pTau in deep-layer CA1 pyramidal cells

(A) Neurons immunoreactive for pTau (AT8, green, arrows) in an aging mouse show nuclear immunoreactivity for CTIP2 (magenta) in CA1d SP. Widefield epifluorescence, case TV136 (14 months).

(B) A pTau+ neuron (asterisk) in distal CA1d SP immunopositive for SATB2 (cyan) in an aged mouse. Note PV+ basket terminals around somata (magenta). Confocal single optical section (0.43 μm thick), case TT21D (23 months).

(C) Weak Wfs1 immunoreactivity (cyan) in pTau+ distal CA1d neurons (AT8, green, asterisks) in a young mouse. Confocal single optical section (1 μm thick), case TV133 (6 months).

(D) Cells in CA1d SP immunopositive for pTau (AT8, green). Lack of detectable CB (cyan) or PV (magenta) immunoreactivity in pTau+ neurons within deep SP (arrows). Widefield epifluorescence.

(E) Electron micrographs of AT8+ filaments (gold-silver particles) within a cell body (left) and an axon terminal (a, right). From an aged mouse (case TT33G, 21-month-old female).

(F and G) Immunoreactivity of SATB2 (cyan) and AT8 (green) in CA1d of aged ntg and tg mice (22 months). Insets, enlarged views of SP. Widefield epifluorescence.

(H) Distribution chart of SATB2+ nuclei counts in CA1d (n = 3 mice/genotype).

Scale bars: 20 μm (A and C); 10 μm (B); 50 μm (D); 0.5 μm (E); 100 μm (F and G). Alv, alveus; d, dendrite; SLM, stratum lacunosum moleculare; SO, stratum oriens; SR, stratum radiatum. See also Figure S3.