Skip to main content
. 2022 Nov 15;41(7):111646. doi: 10.1016/j.celrep.2022.111646

Figure 6.

Figure 6

Identification of hippocampal neurons in relation to pTau

(A) Glass electrode extracellular recordings of pyramidal neurons in freely moving mice. Top, ProS LFP and associated band-pass-filtered spikes from cell TV140h during spontaneous rest and exploration (18 months ntg). Bottom, CA1d LFP (tungsten wire, contralateral) and spikes from cell TV134i (10 months tg). Accel., acceleration. Asterisks, higher-amplitude theta during movement.

(B) Cell TV140h from (A) in ProS (neurobiotin, red) is CB− (cyan) and CTIP2+ (inset, green). Widefield epifluorescence.

(C) Glass electrode recordings in head-fixed tg mice. Wideband LFP of large irregular activity and spikes during rest from cells TV142t (top, 18 months) and BS04a (bottom, 13 months). Right, CS at higher time resolution (asterisks).

(D) Identified CA1 pyramidal cell TV142t (neurobiotin, red) from (C) lacks pTau (green). Widefield epifluorescence.

(E) Identified CA1 pyramidal cell BS04a (neurobiotin, red) from (C) lacks pTau (green) and CB (cyan, asterisk) but is CTIP2+ (green, asterisk). Confocal maximum intensity z-projections (top, 48.5 μm thick; bottom, 9.25 μm thick).

(F) Distribution charts of mean firing rates during rest and movement. Top, pyramidal neurons (rest, p = 0.9452; movement, p = 0.4132); bottom, interneurons (rest, p = 0.9273; movement, p = 0.7765); ntg, gray; tg, green. Points are individual neurons color-coded by age group.

(G) Firing rates for movement versus rest. Right, detail of lower firing cells from left. Scale bars: 100 μm (B, D, left); 50 μm (D, right); 30 μm (E, top); 15 μm (E, bottom).