Figure 4.

Antitumor results of the photodynamic and photothermal effects of Purp@COP. (A) HepG2 cells were treated with or without 50 μg/ml of Purp@COP followed by irradiation with or without 1 W/cm² laser for 10 min and then cultured for 24 h before calcein AM and propidium iodide (PI) staining (scale, 20 μm). (B) Results of dihydroethidium (DHE) staining after HepG2 cells were treated with or without 50 μg/ml of Purp@COP followed by irradiation with or without 1 W/cm² laser for 10 min (scale, 20 μm). (C) Flow cytometry results of Annexin V and PI staining after HepG2 cells were treated with or without 50 μg/ml of Purp@COP followed by irradiation with or without 1 W/cm² laser for 10 min and then cultured for 24 h. (D) Results of crystal violet staining after HepG2 cells were treated with or without 50 μg/ml of Purp@COP followed by irradiation with or without 1 W/cm² laser for 10 min and then cultured for 72 h. (E) The drug absorption of HepG2 cells before or after the culture with different doses of Purp@COP. (F) Protein expression of Bcl-2 of HepG2 cells treated with or without 50 g/ml of Purp@COP followed by irradiation with or without 1 W/cm² laser for 10 min and then cultured for 24 h. Actin was used as a loading control.