Table 8.

Evaluation of the toxicity and physiochemical parameters of the vaccine.

Subunits	Toxicity	PI	Half-life (in vitro)	Half-life (in vivo)	Aliphatic index	Instability index	Molecular weight
HTL epitope	Non-toxin	6.93	5.5 h	2 min	85.10	29.41	10,629.12
CTL epitope	Non-toxin	9.66	1 h	>10 h	85.98	32.70	14,727.00
Linear B-cell epitope	Non-toxin	9.84	3.5 h	>10 h	40.26	43.04	20,466.27
Conformational B-cell epitope	Non-toxin	9.57	1.9 h	>10 h	52.44	34.53	4,503.24
Final vaccine	Non-toxin	9.84	1 h	>10 h	66.01	34.59	59,408.47