FIG. 1.

Restriction maps of the plasmids used in this work. pSGS3 contains a 2,217-bp XbaI fragment carrying the ODC gene of C. albicans (CaODC) in pGEM-T. pAHH1 is a plasmid containing the hisG-URA3-hisG cassette disrupting the ODC open reading frame. pAHH7 and pAHH8 are derivatives of pYPB1-ADHp7 constructed by insertion of the Candida ODC open reading frame under the control of the promoter of ADH1 and by insertion of the Candida ODC gene under the control of its own promoter (thus deleting the ADH promoter), respectively.