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. 1999 Sep;67(9):4870–4878. doi: 10.1128/iai.67.9.4870-4878.1999

FIG. 2.

FIG. 2

(A) Deletion of ODC in C. albicans. The open reading frame of the C. albicans ODC locus is shown. An EcoRI-BglII fragment was replaced by the hisG-URA3-hisG cassette. (B) Southern blot analysis with an ODC fragment from the RsaI-EcoRI fragment as the probe. Genomic DNA samples were digested with BclI (giving rise to a BclI-BclI band of 0.9 kb, an internal fragment of the ODC gene, and a second, ever-present BclI-BclI band [arrow] of 1.3 kb, containing part of the ODC gene and part of the promoter 5′ end). Lanes: 1, CAF4-2 (ura3/ura3 ODC/ODC); 2, ABH1 (ura3/ura3 ODC/odc::hisG-URA3-hisG); 3, ABH2 (ura3/ura3 ODC/Δodc::hisG); 4, ABH3 (ura3/ura3 odc::hisG-URA3-hisG/odc::hisG); 5, ABH4 (ura3/ura3 Δodc::hisG/Δodc::hisG).