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. 1999 Sep;67(9):4870–4878. doi: 10.1128/iai.67.9.4870-4878.1999

FIG. 4.

FIG. 4

Colony growth and morphological characteristics of C. albicans homozygous odc strain at different polyamine concentrations. Column A shows the wild-type strain (CAF 4-2 ura3/ura3), column B shows strain ABH4 (ura3/ura3 Δodc/Δodc) grown at 0.01 mM putrescine, and column C shows strain ABH4 (ura3/ura3 Δodc/Δodc) grown at 10 mM putrescine. Wild-type (CAF4-2) and odc (ABH4) cells were grown in Lee medium, pH 6.7, at 28°C and incubated under conditions that promote germ tube formation and hyphal growth. Row 1 shows colonies of C. albicans cells grown for 7 days at 37°C on solid Lee medium with mannitol. Effects of temperature (37°C), serum (4%), and GlcNAc (1.25%) on germ tube emission are shown in rows 2, 3, and 4, respectively.