FIG. 5.

Resistance to a cell wall-degrading enzymatic complex of C. albicans. The wild type (CAF4-2 [ura3/ura3]) (▴) ABH3 (ura3/ura3 odc::hisG-URA3-hisG/odc::hisG) (▵), and ABH4 (ura3/ura3 Δodc/Δodc) were grown until the exponential phase in Lee medium, pH 6.7, at 28°C with a low (0.01 mM) or a high (10 mM) putrescine concentration, but respective results for low (■) and high (□) concentrations are shown only for ABH4. Aliquots (1.5 × 10⁷ cells) were resuspended in water and treated with 0.025 mg of Zymolyase per ml. The decrease in optical density (OD) (percentage of resistant cells) is plotted as a function of time.