Figure 8.

(A) SFRE synergised with pemetrexed in the inhibition of NSCLC cell proliferation. NSCLC cells were pre-treated with the extract for 3h at a fixed dose of 1/2 IC₅₀ for H1299 and H1975. Combinatory index (CI) according to Chou–Talalay method. (B) SFRE diminished the expression of DHFR and GARFT, in a dose dependent manner. Results are shown in relation to non-treated cells and normalized to the endogenous control B2M. Results are expressed as the mean ± SEM of three independent experiments, each performed in triplicates. Asterisks *, **, *** indicate p-values < 0.05, 0.01 and 0.005, respectively.