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. 2022 Nov 9;10:1049653. doi: 10.3389/fcell.2022.1049653

FIGURE 3.

FIGURE 3

Loss of NRK1 and NRK2 does not affect the transcriptional regulation of denervation-induced muscle atrophy. (A) Timeline of the denervation experimental procedure. Mice were subjected to unilateral resection of the sciatic nerve and tissues were collected 1 week after denervation. (B,C) mRNA expression levels of Nmrk1 (B) and Nmrk2 (C). Gene expression of mRNA extracted from TA muscle measured by qPCR relative to bActin as housekeeping gene. (D) Relative NAD+ content of GC muscle quantified by NAD+ cycling assay. (E) Weight of GC muscle normalized to whole body weight. (F–J) mRNA expression levels of genes regulated by denervation. Results shown are mean ± s. e.m. with *p < 0.05, **p < 0.01, ****p < 0.0001 versus WT, determined by unpaired Student’s t-test, n = 6. CL, contralateral and DV, denervated. Created with BioRender.com.