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. 2022 Nov 9;10:1049653. doi: 10.3389/fcell.2022.1049653

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Copyright © 2022 Sonntag, Ancel, Karaz, Cichosz, Jacot, Giner, Sanchez-Garcia, Pannérec, Moco, Sorrentino, Cantó and Feige.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NRKdKO perturbs MuSC proliferation and differentiation during muscle regeneration. (A) Experimental design of the regeneration time course after cardiotoxin (CTX)-induced muscle injury. (B–E) Representative histological images (B) and quantification of total MuSCs (C), proliferating MuSCs expressing Ki67 (D), and Myogenin-positive cells (E) at 7 dpi. Pax7, red; Ki67, yellow; Myogenin, green; nuclei (Hoechst), blue. Scale bars, 50 µm. Results shown are mean ± s. e.m. with *p < 0.05, **p < 0.01, ***p < 0.001 versus WT, determined by unpaired Student’s t-test, n = 8. Created with BioRender.com.