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. 2022 Nov 9;10:1049653. doi: 10.3389/fcell.2022.1049653

FIGURE 6.

FIGURE 6

NRKdKO delays the regeneration of injured myofibers which recover through compensatory IGF1/AKT signaling. Mice were subjected to unilateral CTX injection into hindlimb muscle, tissues were collected 7 and 14 dpi. (A) Representative images of TA muscle sections stained with laminin (green) and Hoechst for nuclei (blue) at 7 dpi and 14 dpi. Scale bar, 50 µm n = 8. (B) Cumulative distribution of cross-sectional area of regenerating fibers with centralized nuclei at 7 dpi and 14 dpi. (C,D) Representative immunoblot images (C) and quantification (D) by densitometry analysis of Focal Adhesion Kinase (FAK) in WT in NRKdKO muscles, n = 5. (E–I) Representative immunoblot images (E) and quantification (F–I) by densitometry analysis of the AKT pathway in WT in NRKdKO muscles, n = 5. Results shown are mean ± s. e.m. with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus WT, determined by unpaired Student’s t-test (D,F–I) or (B) Kolmogorov-Smirnov test.