Figure 1.

Antiproliferative profile of chromomycins A_5-8 on metastatic melanoma B16-F10 cell line over time. (A), Graphs showing the antiproliferative activity of CA_5-8 after different exposure periods by the SRB assay. Inhibition concentration mean (IC₅₀), total growth inhibition (TGI), and lethal concentration mean (LC₅₀) values in nanomolar (nM) were obtained from interpolation of non-linear regression of normalized absorbance of 3 experiments performed in triplicate. (B), Representative photos of the clonogenic assay, and (C), Number of colonies represented as the mean ± standard deviation. 0.05% DMSO (C-) and 0.6 µM doxorubicin (Dox) were considered as a negative control and positive control, respectively. Statistical differences of treated groups versus C- are expressed as p values indicated above the columns of the groups.