Figure 2.

Chromomycins A_5-8 induce cellular morphological changes and cell death. B16-F10 melanoma cells were treated with DMSO (C-), as a negative control, doxorubicin (Dox), as a positive control, and CA_5-8 (CA5-8) for 24 h. (A) Phase contrast photomicrographs (200x). (B) Representative contour plot graphs of the cell death subpopulation gated in the low forward scatter (FSC) and DAPI⁺ region by flow cytometry. (C) Expression of activated caspase 3 obtained by Western blot. (D) Expression of cleaved PARP1 and γH2AX obtained by Western blot. Values associated with test proteins were normalized to standard α-tubulin for the relative expression measure. (E-G), column graphs of Western blot analysis. Data presented as mean of 3 independent experiments. Flow cytometry analysis was performed in triplicate. ***p value = 0.001 of treated groups compared to the C-.