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. Author manuscript; available in PMC: 2023 Jan 5.
Published in final edited form as: Biochemistry. 2022 Jun 24;61(13):1260–1272. doi: 10.1021/acs.biochem.2c00157

Figure 2.

Figure 2.

PrOF NMR experiment with 40 bp dsDNA binding to 5FW BRD4–BD1. (A) 19F NMR spectra and (B) binding isotherm of 40 bp dsDNA (14–230 μM) with 33 μM 5FW BRD4–BD1 and 25 μM 3FY BRD4–BD1. W81 is the “WPF shelf” tryptophan, and Y97 and Y137 are around the KAc-binding site, colored red and blue, respectively. The change in the chemical shift (Δδ) is shown in purple and is indicated by the dashed line. Kd calculated from the CSP of W81 is reported as the mean ± SD of three experimental replicates.