Figure 2.

Alveolar macrophage frequencies are increased in CD47^−/− mice after influenza virus infection

3 and 7 dpi MФ were analyzed in the lung of WT and CD47^−/− mice.

(A) Mac3⁺ MФ were analyzed by immunohistology 3 dpi (scale bar = 100 μm). Representative slides are shown.

(B) Quantification of Diaminobenzidine⁺ (DAB) cell counts on slides stained for MAC-3 was performed by semi-quantitative analyses. Shown in the diagram are the absolute numbers of DAB⁺ cell counts. Each point represents the mean of ten random, non-overlapping fields of lung tissue from one specimen (n = 3).

(C) Representative dot plots of flow cytometry analysis of CD11c^hi CD11b^lo alveolar macrophages (aMФ) in the lung 3 dpi.

(D) Percentages of aMФ were analyzed by flow cytometry (n = 9).

(E) Total numbers of aMФ were calculated based on flow cytometry analysis (n = 9).

(F) aMФ from WT mice were analyzed regarding their mean fluorescence intensity (MFI) of CD47 and SIRPα 3 dpi (n = 22).

Results are pooled from three or more independent experiments. Data shown are mean ± SEM One-way ANOVA with Tukey’s multiple-comparisons post-test (B), or Student’s t test (D, E, and F) were performed. ∗ = p < 0.05, ∗∗ = p < 0.01.