Figure 3.

Alveolar macrophages are associated with enhanced influenza virus clearance from the lung

MФ populations were depleted by i.n. (aMФ) or i.p. (iMФ) application of clodronate containing liposomes 3 days prior to influenza virus infection.

(A) Representative flow cytometry plots depict the depletion of MФ populations in the lung (3 dpi).

(B) Viral loads were determined by plaque assay 5 dpi. C) aMФ were depleted by i.n application of clodronate liposomes in WT mice and recolonized with aMФ from naive WT or CD47^−/− mice by adoptive transfer. Subsequently, mice were infected with influenza virus and viral titers were assessed by plaque assay 5 dpi.

Data shown are mean ± SEM. Two-way ANOVA with Sidak’s multiple-comparisons post-test (A and B) or Student’s t test were performed. ∗ = p < 0.05, ∗∗ = p < 0.01.