Figure 4.

CD47 restricts the antiviral activity of alveolar macrophages independent of SIRPα

At day 3 after influenza virus infection mice were i.n. treated with pH sensitive fluorescent particles and the phagocytosis of particles by aMФ was determined via flow cytometry after 1 h.

(A) The uptake of fluorescent particles is shown in representative histograms.

(B) The mean fluorescence intensity (MFI) for FITC of aMФ was determined (n = 10).

(C) The viral load in the lung of WT and SIRPα^−/− mice was assessed by plaque assay 7 dpi. Representative results from one of two independent experiments are shown.

Data shown are mean ± SEM. Mann-Whitney test was performed. ∗∗∗∗ = p < 0.0001.