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. 2022 Nov 21;155(1):e202213103. doi: 10.1085/jgp.202213103

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© 2022 Chivet et al.

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Figure 4. — The HTT-Rec mouse line. (A) Mice were injected with tamoxifen to induce the recombination at 6–8 wk of age and were analyzed at variable times thereafter (3, 6, and 10 mo of age). (B) Representative Western blots of HTT and tubulin in TA homogenates of two different CTRL and two different HTT-Rec mice at 3 and 10 mo. (C) Quantification of the relative amount of HTT protein compared with β-tubulin at 3 mo (P < 0.0001, HTT-Rec versus CTRL) and 10 mo (P = 0.0006, HTT-Rec versus CTRL) on eight mice in each group. (D) Real-time qPCR analysis of HTT transcript in quadriceps of CTRL and HTT-Rec mice at 3 mo (P = 0.0235, HTT-Rec versus CTRL) and 10 mo (P = 0.0074, HTT-Rec versus CTRL, n = 3–4 mice in each group), compared with β-actin, HPRT, and GAPDH as reference genes. The amount in CTRL littermate was set to 1. The quantification was performed using the ΔΔCt method. Graphs represent mean ± SEM. Statistical analysis: unpaired t test. Source data are available for this figure: SourceData F4.