Table 1.
Primers for genotyping | ||
---|---|---|
Forward (5′-3′) | Reverse (5′-3′) | |
Cre-ERT2 | ATTTGCCTGCATTACCGGTC | ATCAACGTTTTCTTTTCGGA |
HTT-Flox | CTAAAGCGCATGCTCCAGACTG | AGATCTCTGAGTTATAGGTCAGC |
HTT-WT | CATTTGATTCTTACAGGTAGCCTG | AGATCTCTGAGTTATAGGTCAGC |
Primers for qPCR | ||
HTT | TGAGAGTCAGTGTGAAGG | CTACTGAGGATGGAGTAGAC |
β-Actin | GACAGGATGCAGAAGGAGATTACTG | CTCAGGAGGAGCAATGATCTTGAT |