Skip to main content
. 2022 Nov 21;155(1):e202213103. doi: 10.1085/jgp.202213103

Table 1.

Primers for genotyping

Primers for genotyping
Forward (5′-3′) Reverse (5′-3′)
Cre-ERT2 ATT​TGC​CTG​CAT​TAC​CGG​TC ATC​AAC​GTT​TTC​TTT​TCG​GA
HTT-Flox CTA​AAG​CGC​ATG​CTC​CAG​ACT​G AGA​TCT​CTG​AGT​TAT​AGG​TCA​GC
HTT-WT CAT​TTG​ATT​CTT​ACA​GGT​AGC​CTG AGA​TCT​CTG​AGT​TAT​AGG​TCA​GC
Primers for qPCR
HTT TGAGAGTCAGTGTGAAGG CTA​CTG​AGG​ATG​GAG​TAG​AC
β-Actin GAC​AGG​ATG​CAG​AAG​GAG​ATT​ACT​G CTC​AGG​AGG​AGC​AAT​GAT​CTT​GAT