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. Author manuscript; available in PMC: 2022 Nov 23.
Published in final edited form as: J Vis Exp. 2022 Jun 8;(184):10.3791/64003. doi: 10.3791/64003

Figure 2: Quantification of staining intensity for brush border and adherens junction proteins following 24 h lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-α), and hypoxia treatments.

Figure 2:

(A) Villin (red) levels compared among treatments; a = p < 0.0001 compared to control; b = p < 0.05 compared to control; c = p < 0.0001 compared to hypoxia; d = p < 0.05 compared to hypoxia; e = p < 0.001 compared to TNF-α; f = p < 0.01 compared to LPS + hypoxia; g = p < 0.0001 compared to LPS; and (B) E-cadherin (green) levels compared among treatments; a = p < 0.0001 compared to control; b = p < 0.0001 compared to hypoxia; c = p < 0.01 compared to LPS; d = p < 0.01 compared to TNF-α; e = p < 0.05 compared to LPS + hypoxia. Significance was assessed using one-way analysis of variance (ANOVA) with Tukey's test for post-hoc analysis, presented as mean ± standard error of the mean (SEM) (n ≥ 7/group). Control represents normoxia with PBS as vehicle control.