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The expression of WT increased the binding between SURF4 and SAVI. (A) HEK293T cells were transfected with the plasmids as indicated. Cell lysates were prepared, and FLAG-STING was immunoprecipitated with anti-FLAG beads. The cell lysates and the immunoprecipitated proteins were analyzed by western blot. (B) A graphical abstract illustrating the three types of STING complex with their subcellular localizations.
